N-METHYLENE PHOSPHONIC CHITOSAN: A NOVEL SELF- ASSEMBLED TEMPLATE FOR ENAMEL REMINERALIZATION
Journal: Asian Pacific Journal of Microbiology Research (AJMR)
Author: Kun Tian, Qin Du, Chuhang Liao, Wei Fei
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
To evaluate the feasibility of phosphonic chitosan membrane in guiding enamel remineralization. We design a organic molecules model to induced the crystallization of hydroxyapatite with 3D-structure in a controllable way under dentin surface in vitro. N-methylene phosphonic chitosan ( NPCS) was synthesized via formal dehyde addition and condensation with phosphoric acid in a step reaction. The cross-linking reaction between the collagen on the dentin surface and phosphonic chitosan guided by phosphate ions dental adhesive agent. CaCl2 and Na3PO4-12H2O solutions were added after the crosslinking process. We observed that hydroxyapatite nanorods can be controlled followed by in situ crosslinking process and triggered by conditions of pH and ionic strength. The dentinal tubule were blocked by neonatal hydroxyapatite layer and this composite a continuous structure of columns crystal with size of 10-20nm. The results showed that phosphonic chitosan monolayer can be used as a potential effective modifier in inchoate enamel remineralization.